Specificity of amplification. A genome segment of pPVA (W) and pPVA-Mu (I) spanning the region between nt 5152 and nt 6375 was subjected to insertion-specific amplification reactions with the primer pairs INS1/FO12R (FO12R: 5′-AGCCTGATATCAGTATAGGGACTC) and INS1/FO13R (FO13R: 5′-ATAGGGACTCTCATCTAGTGTGTAC). Products from the former reaction were radioactively labeled with the primer INS2 (INS2: 5′-TATACTCTTCAGATGCGGCCGCA, with insertion-specific nucleotides underlined) or FO12R. Products from the latter reaction were labeled with the primer FO13R. Insertions and primers are illustrated by triangles and arrows, respectively. The radioactive labels are indicated by asterisks. (A) Labeled products were separated by urea-PAGE gel. Numbers to the right refer to the band pattern in lane5 and indicate the positions of insertions in the PVA genome. Sample numbers are indicated below each lane.
