Comparative Analysis of Chloroplast Genomes: Functional Annotation, Genome-Based Phylogeny, and Deduced Evolutionary Patterns

Table 3.

Proteins Annotated as ORF or unknown which Are Assigned to Proteins of Known Function

μ-score Synechocystisproteins Chloroplasts proteins—ORF or unknown PSI- BLAST
Prot. ID Prot. function Gene aa Prot. ID Prot. function Organism aa Hits Prot. detected
1 17.10 6466376 rubisco expression prot cfxX/Q 301 1276694 ORF301 Porphyra purpurea 301 >1000 CFXQ_CYAME
2 12.00 sll1536 molybdopterin biosynthesis prot MoeB moeB 392 1276801 ORF382 Porphyra purpurea 382 585 moeB_MYCTU
3 9.60 sll1536 molybdopterin biosynthesis prot MoeB moeB 392 6466397 chlN (incorrect) Cyanidium caldarium 395 574 moeB_MYCTU
4 7.60 sll0621 thiol:disulfide interchange prot DsbD dsbD 248 1276830 ORF240 Porphyra purpurea 240 58 cycZ_ECOLI
5 5.40 sll1035 uracil phosphoribosyl transferase upp 216 1276820 ORF198 Porphyra purpurea 198 88 UPP_STRSL
6 4.70 slr1365 BioY prot bioY 217 1016197 ORF188 Cyanophora parado 188 26 BIOY_BACSH
7 3.90 slr0839 ferrochelatase hemH 387 1276821 ORF48 Porphyra purpurea 48 47 HEMZ_SYNY3
8 3.60 slr1365 BioY prot bioY 217 7259544 unknown Mesostigma viride 223 2 bioY_SYNY3
9 2.50 11691 cytochrome b6/f subunit VI (3.5kD) petL 31 5881712 ORF31 Arabidopsis thaliano 31 2 PETL_BETVU
10 2.50 12005 cytochrome b6/f subunit VI (3.5kD) petL 31 1262631 ORF62b Pinus thunbergii 62 2 PETL_BETVU
11 2.50 11809 maturase K matK 509 295955 ORF506 Euglena gracilis 506 994 mat_PSEAL
  • List of ORFs from different cpDNAs for which functional annotation has been possible. The results from PSI-BLAST are also shown, indicating the number of significant hits obtained (column 11), and the ortholog assigned (column 12). Proteins in column 12 are labeled according to SwissProt nomenclature.

  • Not in Synechocystis.

This Article

  1. Genome Res. 12: 567-583

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