Table 3.

Screening of the Treponema pallidum BAC Genomic Library with Pooled Rabbit Sera

Clone	Antigen identified	Possible ORFs	Most probable ORF	Function of predicted antigen
TP085	55 kDa	0024–0034	TP0030	GroEL—heat shock protein 60 (58kDa)
TP021	68 kDa	0208–0216	TP0216	DnaK—heat shock protein 70 (68kDa)
TP029	38 kDa	0253–0259	TP0257	GlpQ—glycerophosphoryldiester phosphodiesterase (41 kDa)
	33 kDa	0304; 0317–0323	TP0319	TmpC—membrane lipoprotein (37.8 kDa)
TP201	30 kDa	0860–0864	TP0862	peptidyl-prolyl cis-trans isomerase, FKBP-type (28 kDa)
TP076	36 kDa	0965	TP0965	membrane fusion protein, putative (35 kDa)
TP094	47 kDa	0574–0595	TP0574	carboxypeptidase (48 kDa)
TP003	90 kDa	0429–0437	TP0435	lipoprotein, 17 kDa (tpp 17)
	55 kDa	0477–0486	TP0486	antigen, p83/100 (53 kDa)
TP173	75 kDa	0747–0755	TP0748	CfpA—cytoplasmic filament protein A (79 kDa)
	43, 35, 25	0768–0773	TP0768	TmpA—membrane protein (37 kDa)
	kDa		TP0769	TmpB—outer membrane protein (37 kDa)

↵GroEL is closely related to heat shock protein homologs from more than 60 different bacteria.
↵Sequence of DnaK proteins are highly conserved among many organisms, e.g., T. pallidum DnaK is more than 58% identical to E. coli DnaK (Bardwell and Craig 1984).

BAC Library of T. pallidum DNA in E. coli