Novel Fluorescence Labeling and High-Throughput Assay Technologies for In Vitro Analysis of Protein Interactions

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Figure 2.
Figure 2.

Effect of the linker structure on the yields of labeled Jun proteins. Fluorescein (A), RhG (B), or Cy5 (C) linked to puromycin through various linkers was added to the in vitro translation reaction mixture at various dye concentrations. The products were analyzed by 16.5% Tricine-SDS-PAGE and quantified with an imaging analyzer.

This Article

  1. Published in Advance February 15, 2002, doi: 10.1101/gr.218802 Genome Res. 12: 487-492

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