High-Density Cell Microarrays for Parallel Functional Determinations

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Figure 3.
Figure 3.

S. cerevisiae cell microarrays for assaying the drug target of rapamycin. Ninety four yeast homozygous deletion strains, includingfkb1 strain and two negative controls, were arrayed with five repeats in a total of 576 nanocraters. fkb1 strain was arrayed at 8A to 8F; two negative controls that did not contain any cells were arrayed at 15M to15R and 18S-18X. One set of cell microarrays was grown on YPD and the other on YPD containing 1 μg/mL of rapamycin at 30°C overnight.

This Article

  1. Published in Advance February 15, 2002, doi: 10.1101/gr.213002 Genome Res. 12: 482-486

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