Figure 1.
Schematic diagram of coupled fabrication of cell microarrays. (A) A robot-controlled pin with cells on the tip was programmed to strike the cellulose ester membrane to form nanocraters and simultaneously inoculate cells into them. The cellulose ester membrane was placed on top of a cushion during the arraying process. (B) Top view of arrayed nanocraters. The diameter of the nanocraters was 125 μm with a depth of 10–125 μm. The distance between centers of the adjacent nanocraters was 375 μm. (C) Cell microarray membranes were incubated on the surface of agar or liquid medium. (D) Nanocraters with yeast cells at their bottoms.
