Domain-Level Differences in Microsatellite Distribution and Content Result from Different Relative Rates of Insertion and Deletion Mutations

Table 2.

Growth Rate of Mutants Does Not Appear to Be Controlled by the Direction of the Analyzed Microsatellite Mutations

Competition Microsatellite length Initial mixture Time in competition Final mixture Relative growth
1 11 (single deletion) 1 4 days 2 1
13 (single insertion) 2.5 48 9.6
2 10 (double deletion) 1 1 day 16 8
14 (double insertion) 1 2 1

  • Competition experiments between mutants with different microsatellite lengths. Initial mixture ratios were determined by plating separate pre-competition cultures at a 10−6dilution on plates and counting colonies. Final mixture ratios were determined by plating the mixes after the stated “time in competition” and sequencing the microsatellite locus from random colonies.

  • ↵Includes several colonies that carried microsatellites longer than 13 repeats (these are most likely derived through mutation from the clone with 13 repeats). These included 14 repeats (4), 15 repeats (3), and 16 repeats (2).

This Article

  1. Genome Res. 12: 408-413

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