(A) ClustalW sequence alignment of motif MDS00113. (The SWISS-PROT/TrEMBL accessions of the sequences are as follows: (HSA)DKFZP434B1517, Q9UFF7; (HSA)IMAGE4304203, AAH09972; (HSA)FLJ11937 [FLJ21739], Q9H6X6; (HSA)FLJ11937, Q9HAA1; (HSA)FLJ00109, Q9H7I0; DMEL)CG15609, Q9V7X1; (HSA)KIAA1668 [FLJ14966], BAB55422; (MMU)4921517J23Rik, Q9D5U9; (HSA)KIAA0819 [fragment], O94909; (DMEL)CG11259 [variant], AAK93415; DMEL)CG11259, Q9VU34; (MMU)EST57332, 4930438G05; (MMU)EST57332 [variant], 4933434L05; (HSA)KIAA1668, Q9BY92; (HSA)KIAA1668 [Unknown], Q9BVL9; (HSA)DJ1014D13.3, Q9UH43; (HSA)FLJ23471, Q9H5F9; (RNO)Fra1, P10158 (HAS)FRA1 [variant], CAC50237; (HSA)FRA1,P15407; and (MMU)Fra1, P48755. The putative translations of segment EST573322 and its variant (clone ID 4930438G05 and 4933434L05) were taken directly from the FANTOM1 database because the sequence has no DDBJ accession number. (B) Topology-based multiple sequence alignment of 13 MDS00113 representatives. Regions that were predicted as coiled coil were aligned in the upper part. The lower part of the alignment contains the noncoiled regions. The positions of motif MDS00113 and leucine heptad repeats, the predicted coiled coil regions, and the coiled coil trigger sequence (xxLExchxcxccx) are indicated. Letters a to g indicate helix positions. NLS symbolizes the nuclear localization signal. (C) Domain map of MDS00113 containing putative proteins. The abbreviations and InterPro accessions are as follows: M, MDS00113; L, leucine heptad repeat; N, nuclear localization signal; C, coiled coil; CH, calponin-homology domain, IPR001715; LIM, LIM domain, IPR001718; DER, delayed-early response, IPR002259; B, basic-leucine zipper, IPR001871; PNDO, pyridine nucleotide-disulfide oxidoreductase; PRR, proline-rich region, IPR000694; and L7/12, ribosomal L7/L12 C-terminal domain, IPR000206.
