Figure 7.
Top: reference images from three different hybridizations, all processed to display the same absolute pixel ranges. Bottom: cumulative proportions of standard deviations of the log2ratios of the replicate spots for each hybridization when analyzed using explicit spot segmentation and spot identification based on a circularity assumption. The three hybridizations differ substantially in average signal intensity, with the leftmost image having the lowest signal and the rightmost having the highest. We see the effect predicted in Fig. 2, with higher signal decreasing the effect of pixel misidentification.
