V-SINEs: A New Superfamily of Vertebrate SINEs That Are Widespread in Vertebrate Genomes and Retain a Strongly Conserved Segment within Each Repetitive Unit

Table 1.

The Relative Contributions of V-SINEs to the Genomes of Various Organisms

Organism Percent of genome Reference
Sharks and rays ∼10–0.01 Ogiwara et al. 1999
Zebrafish ∼10 Izsvák et al. 1996
Shimoda et al. 1996
Izsvák et al. 1997
Catfish ∼0.3 Kim et al. 2000
Rasbora a ∼ 0.004 This study
Lamprey b ∼ 0.04 This study
Lungfish c ∼ 0.15 This study
Medaka d ∼ 0.1 This study
Shimoda et al. 1996
Izsvák et al. 1997
Fugu e ∼ 0.02 This study
Izsvák et al. 1997
  • Copy numbers were estimated as follows: (a) When 10,000 clones, which represented about 3 × 104 kbp of rasbora genomic DNA, were screened with the Ras1 SINE, four clones, which represented about 1.2 kbp of Ras1 SINEs, were isolated by hybridization. (b) 5,000 clones, which represented about 1.5 × 104 kbp of lamprey genomic DNA, were screened with the Lam1 SINE, and 25 clones, which represented about 6.3 kbp of Lam1 SINEs, were isolated by hybridization. (c) 5,000 clones, which represented about 1.5 × 104 kbp of lungfish genomic DNA, were screened with the Lun1 SINE, 75 clones, which represented about 23 kbp of Lun1 SINEs, were isolated by hybridization. (d) The number of Ac1 SINEs in the medaka genome was estimated from the intensity of signals on a Southern blot (data not shown). (e) In fugu, about 3.5 kbp were identified as Ac1 SINEs in the 2 × 104 kbp of the fugu genome that have been sequenced to date (http://fugu.hgmp.mrc.ac.uk).

This Article

  1. Genome Res. 12: 316-324

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