Control Genes and Variability: Absence of Ubiquitous Reference Transcripts in Diverse Mammalian Expression Studies

Table 1.

Traditional control genes across four datasets

Gene Description NC160 AML/ALL Huge index TPA
MFC CV MFC CV MFC CV MFC CV
ACTB Actin, beta 7.8 0.39 3.5 0.32 1.3 0.11 4.6 0.35
ALDOA Aldolase A 8.2 0.37 5.7 0.48 2.9 0.37 2.9 0.31
G6PD Glucose-6-phosphate dehydrogenase 7.7 0.90 5.0 0.45 2 0.28 4.6 0.43
GAPD Glyceraldehyde-3-phosphate dehydrogenase 5.3 0.31 12.3 0.33 2 0.19 1.9 0.17
B2M Beta-2-microglobulin 25.6 0.58 14.0 0.42 3.4 0.27 4.6 0.49
PFKP Phosphofructokinase, platelet 12.4 0.68 >12 1.66 >96 0.08 14.0 0.56
PGK1 Phosphoglycerate kinase 1 6.5 0.44 6.8 0.36 N/A N/A 4.9 0.41
PGAM1 Phosphoglycerate mutase 1 (brain) 5.2 0.40 12.4 0.48 1.6 0.19 3.6 0.47
TUBA1 Tubulin, alpha 1 (testis specific) >50 1.09 53.7 0.78 3.1 0.84 6.7 0.46
HPRT1 Hypoxanthine phosphoribosyltransferase 1  (Lesch-Nyhan syndrome) 10.7 0.45 43.9 0.53 1.6 0.42 5.6 0.49
VIM Vimentin >300 0.68 12.7 0.42 3.8 0.41 28.9 0.94
LDHA Lactate dehydrogenase A 91.0 0.38 9.2 0.40 5.5 0.60 4.1 0.34
  • CV, coefficient of variation; MFC, maximum fold change; AML/ALL, acute myeloid leukemia/acute lymphoblastic leukemia panels; TPA, phorbol 12-myristate 13-acetate induction.

This Article

  1. Genome Res. 12: 292-297

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