Protein–Protein Interactions Between Large Proteins: Two-Hybrid Screening Using a Functionally Classified Library Composed of Long cDNAs

Table 1.

Summary of Experimental Results

Description Total
Number of baits assessed 71
Number PCR products obtained 65
Number of baits cloned 64
Self-activation 12
Number of baits that underwent two-hybrid screening 52
Number of baits identified to have interactions 32
Total number of protein pairs exhibiting interactions 84
Interaction identified multiple times 42
  • Self-activation was assessed on SD/−Ade/−His/−Trp plates.

This Article

  1. Genome Res. 12: 1773-1784

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