Reassessment of interactions after exchanging cDNAs in baits and preys via the Gateway system. Image of yeast two hybrids grown on selection media. After exchanging the plasmids contained in the original bait and prey using the Gateway system, new bait plasmids (KIAA0777, KIAA1256, KIAA1296, or KIAA1496), new prey plasmid (KIAA1368), and an empty vector (pACTGW-attR) were transformed into yeast AH109. The viability of the transformants was assessed on SD/−Leu/−-Trp and SD/−Ade/−His/−Leu/−Trp plates. The + symbol located in the right-hand column indicates that the MEL1 gene product, α-galactosidase, is actively expressed. Blue-colored growth on nutritional selection plates in the presence of X-α-Gal indicates active expression of α-galactosidase.
