Protein–Protein Interactions Between Large Proteins: Two-Hybrid Screening Using a Functionally Classified Library Composed of Long cDNAs

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Figure 2.
Figure 2.

Yeast two-hybrid screening and subsequent determination of interacting domains. The domain organization for bait (left) and prey (right) KIAA proteins are shown. Blue bars indicate independent clones (one bar represents one prey). The length of the blue bar indicates the sequence of the KIAA protein contained within an isolated prey. The interacting domains located in the bait and preys are indicated by pink (bait) and green (prey) brackets. In the case of KIAA1634, we estimated that KIAA1132 can bind two different domains: the first and second PDZ domain, as shown.

This Article

  1. Published in Advance October 15, 2002, doi: 10.1101/gr.406902 Genome Res. 12: 1773-1784

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