BAC Reverse Northern blot of BAC clones containing differentially expressed genes. BAC DNA was digested with PstI, the fragments were subcloned into pBluescript SK+, linearized with NotI, and agarose gel size-fractionated in four replicate gels. After transfer to charged nylon membranes, each membrane was hybridized with heterologous liver cDNA from one baboon in the sib-pair on a specified diet. The HDL phenotype L indicates the low HDL responder and the H indicates the high HDL responder of the sib-pair. The diet is denoted by B for basal and C for challenge. The BAC clone number in the contig is indicated on the bottom line of the figure. DNA fragments pursued in subsequent expression array and sequencing experiments are boxed and assigned a letter (a, b, c, d, and e).
