Figure 1.
Schematic outline for analysis of DNA methylation based on oligonucleotide microarray. Genomic DNA is bisulfite treated and amplified by PCR for a specific CpG island region of interest. The amplified product is labeled with Cy5 fluorescence dye and hybridized to oligonucleotide probes attached to a glass surface. At leftan oligonucleotide probe is designed to form a perfect match with a target DNA containing the unmethylated allele. At right a probe is designed to form a perfect match with the methylated DNA target.
