Transcription termination sites of Arabidopsis thaliana Athilagroup elements. RNA was isolated from a methylation-deficient strain (ddm1) and amplified by RT-PCR using anAthila4 primer and a poly(T) primer/adaptor. PCR products were cloned and sequenced. At the top of the diagram is a genericAthila LTR with the region denoted wherein the transcripts terminate. Clones are listed that match the Athila6 andAthila4 elements. The numbers next to the arrows indicate the base position for transcription termination sites within the LTR. Clones were recovered with variable termination sites, as indicated by the gaps in the alignment. Three clones (pDW820, pDW825, and pDW780) match highly degenerate, unclassified Athila elements, and the numbers for the BACs that carry these elements are provided.
