Table 2.
Sequences of Oligonucleotide Primers and Probes Used for Herpesviral DNA Polymerase Consensus and Specific PCRs, Southern Blot Hybridizations and mtDNA PCR Amplification
| Oligonucleotide | Orientation | 5′–3′ sequence |
| DNA pol degenerate primers | ||
| DFASA | + | GTG TTC GAC TTY GCN AGY YTN TAY CC |
| VYGA | + | ACG TGC AAC GCG GTG TAY GGN KTN ACN GG |
| GDTD1B | − | CGG CAT GCG ACA AAC ACG GAG TCN GTR TCN CCR TA |
| P2s | + | GAG TTT CCK TCG GAR TAY GAC ATG |
| PanRHV2 specific primers | ||
| Pan2as | − | TCA CTT AAT GCG GTT GGA TCT AG |
| P2eas | − | CAT TGC GTG TGA CCT TGA TG |
| P2ias | − | TCT GTT TTC GCT TGC TCA AC |
| Pp2es | + | GCA TCT TTA TCA TAC GCT AAC GG |
| Pp2is | + | CGT TTG TTC TCA GTG GAG GAA AG |
| Pp2as | − | ATG GTG CGT CCC TGG AGC |
| Specific probes | ||
| PanRHV2-1 | + | CTG CAT TAC CTG TTG TCC TAA CGC C |
| PanRHV2-2 | + | ATA TGG TTT TAC TGG AGT AGC CAA CG |
| PanRHV1a | + | GTG GGT CTA CTG CGG AAG CCT ATA AAC GTC TC |
| PanRHV1b | + | TCT GTG CTT CTA CAT AGA CCA ATT GAG ACA CA |
| GorRHV1 | + | GCG AGT CTC CTG CAA AAG CCC ATA GAC GTT CCC A |
| mtDNA primers | ||
| MTD1S | + | CAC CAT TAG CAC CCA AAG CT |
| MTD1AS | − | CCT GAA GTA GGA ACC AGA TG |
-
↵Degenerate oligonucleotide primers described (Rose et al. 1997).
-
+, sense; −, antisense.
-
Positions of degeneracy are given. N = A, C, G and T; Y = C and T; R = A and G; K = G and T.
