
A three-way CLUSTALW alignment of GAL4 promoter sequences reveals strong conservation of known regulatory elements. The entire promoter is shown in the alignment, from the stop codon of the upstream gene (YPL247C) to the ATG start codon ofGAL4. Regulatory elements defined by Griggs and Johnston (1993) are boxed and labeled: UAS (upstream activator sequence), UES (upstream essential sequence), and Mig1 binding sites. Transcriptions start sites are depicted as arrows.











