Figure 4.
Effect of exonuclease-resistant random primers on amplification. Annealing reactions (20 μL) were performed as described except that the template DNA was M13 double-strand RF DNA and the primers were either exo-resistant or exo-sensitive hexamers. Reactions contained 1 ng of M13 DNA with exo-resistant or exo-sensitive primers and φ29 DNA polymerase as indicated. Reactions were incubated at 34°C for 13 h. DNA synthesis was quantitated by the incorporation of radioactive nucleotide.
