Rapid Amplification of Plasmid and Phage DNA Using Phi29 DNA Polymerase and Multiply-Primed Rolling Circle Amplification

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Figure 2.
Figure 2.

Comparison of amplification efficiency between singly- and randomly-primed M13 single-strand circular DNA. Rolling circle reactions were performed as described and contained either 1 ng of singly-primed single-strand M13 DNA or 1 ng of random hexamer-primed single-strand M13 DNA. Reactions were incubated at 34°C for 24 h and aliquots were taken to measure DNA synthesis as indicated.

This Article

  1. Genome Res. 11: 1095-1099

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