Gene Structure Prediction and Alternative Splicing Analysis Using Genomically Aligned ESTs

Shown here is the distribution of various types of splice pairs in different functional regions. REF, number of known splice junction pairs. REF^alt, known splice pairs affected by alternative splicing. REF^est, known splice pairs found in ESTs. REF^alt, est, known splice pairs that are affected by alternative splicing and found in ESTs. ALT, alternative splice pairs mutually exclusive with known splice pairs. NEW, alternative splice pairs inserted into a known exon. One alternative splice pair (ALT) can be mutually exclusive with multiple known splice pairs (REF^alt), and vice versa. Hence, 575 alternative splice pairs affected 639 known splice pairs. It is worth noting that two alternative splice pairs span all three functional regions.