Production and Sequencing of Bovine EST Libraries
| cDNA Library | Tissue | Collection animal | % of RNA | Fold normal | Avg insert length (bp) | No.of seq | % GenBank nr match | % Unique |
| MARC 1BOV | Mesenteric lymph node | 3 | 18 | 18.3 | 1300 ± 632 | 23,470 | 18 | 63 |
| TIGR library ID: 2819 | Hilar lymph node | 3 | 18 | (n = 500) | ||||
| Post-pubertal ovary | 4 | 18 | ||||||
| Kidney-associated fat | 3 | 7 | ||||||
| Hypothalamus | 3 | 18 | ||||||
| Pituitary | 2 | 18 | ||||||
| Subcutaneous fat | 3 | 3 | ||||||
| MARC 2BOV | Testis | 1 | 15 | 16.8 | 1143 ± 594 | 16,494 | 23 | 65 |
| TIGR library ID: 2820 | Thymus | 2 | 16 | (n = 139) | ||||
| Semitendenosous muscle | 2 | 15 | ||||||
| Longissimus muscle | 2 | 16 | ||||||
| Pancreas | 2 | 6 | ||||||
| Adrenal gland | 2 | 16 | ||||||
| Endometrium | 4 | 16 | ||||||
| MARC 3BOV | Bone marrow | 2 | 21 | 14.6 | 1188 ± 564 | 6,196 | 23 | 82 |
| TIGR library ID: 2821 | Alveolar macrophage | 3 | 20 | (n = 310) | ||||
| Pre-pubertal ovary | 3 | 19 | ||||||
| Fetal semitendonosous muscle | 5 | 20 | ||||||
| Fetal longissimus muscle | 5 | 20 | ||||||
| MARC 4BOV | whole embryos (pool of 4) | d20 embryos | 50 | 12.7 | 1008 ± 879 | 22,360 | 20 | 60 |
| TIGR library ID: 2822 | whole embryos (pool of 2) | d40 embryos | 50 | (n = 232) | ||||
| Total sequences | 68,520 |
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RNA from the indicated tissue was collected from one of five animals, with the exception of the d20 and d40 embryos which each represented a pool of embryos. The five animals used were (1) a 48-h-old Piedmontese-Angus cross calf, (2) a 20-d-old Simmental-MARCIII calf, (3) an 800-lb MARCIII yearling heifer, (4) a pregnant six-year-old Limousin cow, or (5) a d100 fetus from a Gelbvieh-Limousin cross. A pool of 5 ug of mRNA was used to construct each library, with percentage of the total amount of RNA from each tissue as indicated (% of RNA). Effective normalization of each library (fold normal) is expressed as the fold decrease in abundance of EF1a clones. The number of EST sequences deposited in GenBank from each library is indicated. Sequences were compared to GenBank nr database via BLASTanalysis, and percentage of clones showing significant match (score >300) is shown. The percentage of sequences with no significant overlap with other sequences from the same library is indicated (% unique).
