Figure 2.
(A) Results from amplifications of the human K-rasgene. Tenfold serial dilutions of human DNA comprising 105–10–2 genomes per reaction, as well as no template controls (NT) were thermocycled and fluorescence was recorded during the annealing step of each cycle. The ΔRn values represent fluorescence at each cycle, minus the average baseline signal calculated between cycles 3–15, as generated by the default settings on the ABI 7700 instrument. (B) A plot of cutoff threshold (Ct) value vs. input template concentration.
