A Bacterial Artificial Chromosome Library for Sequencing the Complete Human Genome

(Downloading may take up to 30 seconds. If the slide opens in your browser, select File -> Save As to save it.)

Click on image to view larger version.

Figure 3.Figure 3.
Figure 3.

A 1.5-Mb BAC contig map localized on chromosome 14q24.3. A total of 169 BAC clones were identified from the RPCI-11 BAC library. The contig has been assembled according to the hybridization results using SEGMAP. The deduced markers are depicted with a black circle along the top, and each short horizontal line with black circles represents a BAC clone. Combination of the plate number and well position represents the clone name from the RPCI-11 library. For example, 1035K4 is found in plate 1035 at well position K4. The markers derived from the T7 or SP6 vector end are condensed as −T or −S after the clone name. The size of each clone is indicated in kilobases in parentheses. Markers have arbitrarily been assigned even spacing for diagrammatic purposes, and the length of the horizontal lines does not accurately represent the insert size. Only 121 markers derived from BAC ends are shown in this figure. Note that seven markers (between 289P13-S and 466E15-T) are overlapping in two contiguous figures. BACs derived from MboI library section (segment 5) are shown in red.

This Article

  1. Genome Res. 11: 483-496

Preprint Server