20p12 BAC/PAC array. (A) Ideogram of the invdup(20p) marker chromosome showing the inverted duplication breakpoint in band 20p11.2 (arrow) and the activation of a neocentromere at 20p12. p′ and q′ indicate the short and long arms of the marker chromosome relative to the neocentromere, respectively. (B) BAC/PAC array constructed from public databases around the neocentromere. Distances (in Mb units) within the array are plotted relative to PAC dJ811H13 on the p′ arm of the marker chromosome. The size of each horizontal bar is proportional to the corresponding insert size of the BAC or PAC. Addresses of the BAC/PACs are listed on the right. The prefix “dJ” denotes a PAC while “bA” indicates a BAC. PACs dJ1009E24 and dJ905G11 map outside the array and are indicated by broken lines. BAC/PACs that were not used in the CIA analysis (see Methods) are shown in italics and recessed. Shaded box represents the CENP-A binding region (see Results).
