Fluorescence Polarization in Homogeneous Nucleic Acid Analysis II: 5′-Nuclease Assay

Table 3.

Concordance Rate of Genotypes Called by FRET or FP Reading of the 5′-Nuclease Assay for 20 Markers

Marker No. of samples tested No. of PCR failures No. of confident calls Percent concordance
D4S2341 90 0 90 100
D10S1188 90 1 89 100
D4S1301 90 0 90 100
D111964 90 0 88 100
D7S8 90 0 90 100
D10S1257 90 0 90 100
WIAF1177 90 0 90 100
WIAF1100 90 0 90 100
WIAF1244 90 0 90 100
WIAF1296 90 0 90 100
WIAF1430 90 1 89 100
WIAF1504 90 0 90 100
WIAF1842 90 0 90 100
WIAF1634 90 0 90 100
WIAF1761 90 1 89 100
WIAF2081 90 0 90 100
WIAF2117 90 0 90 100
WIAF2188 90 0 89 100
WIAF270 90 0 90 100
WIAF2072 90 0 89 100

This Article

  1. Genome Res. 11: 436-440

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