Flow of clones, sequences, and information in the German cDNA Consortium. 5′ EST sequences were systematically generated from the clones of 384-well microtiter plates and analyzed for hits in public databases. Clones with novel sequences were 3′-EST sequenced and these ESTs were analyzed again for novelty. Clones of uncharacterized transcripts were reported back to the sequencers who then did the full-length sequencing of cDNAs. The final sequence was analyzed comprehensively with bioinformatic tools and the outputs were evaluated manually. The clones feed functional analysis projects that take advantage of the clone resources generated.
