CAE microplate multiplex SNP genotyping analysis of three HFE variants. (A) Separation of amplicons generated from a wild-type sample. The three wild-type ET-ROX (red) labeled AS amplicons are found at 174 bp (63H-187c), 211 bp (65S-193a), and 223 bp (282C-845g). The other ET-ROX (red) PCR controls are found at 244, 268, 281, 293, and 363 bp. The inset presents the raw unprocessed data. The 528 bp control is marked in this raw data but is not displayed in the analyzed data because it is outside the sizing standard range. The 20 TET-labeled sizing standards are detected in the green channel. (B) Separation of the amplicons generated from an S65C heterozygote sample with PCR controls labeled with ET-TAM (black). The presence of the 65C-193t mutant allele in this sample appears as an extra peak labeled with ET-R110 (blue) at 201 bp. (C) Separation of the amplicons generated from an H63D/C282Y compound heterozygote sample. Note the presence of the extra ET-R110 (blue) peaks at 160 and 232 bp, generated by the presence of the 63D-187g and 282Y-845a mutant alleles, respectively.
