Identification of Human Epidermal Differentiation Complex (EDC)-Encoded Genes by Subtractive Hybridization of Entire YACs to a Gridded Keratinocyte cDNA Library

Table 2.

Characterization of EDC Genes Encoding Known Proteins

YAC probe Detected transcript (gene) Size of cDNA Homologous to: acc. no. (identities) Positive YACs
Established EDC genes
Involucrin 2139 bp M13902 /M13903 874_d_5, 986_e_10, 890_e_4,
(IVL) (99%)  955_e_11
Small proline-rich protein 3 890 bp / 859 bp AF077374 874_d_5, 986_e_10, 890_e_4,
(SPRR3) (99% / 95%)  955_e_11, 692_c_1
986_e_10 Small proline-rich protein 1A/1B 769 bp / 595 bp L05187 (99%) / 874_d_5, 986_e_10, 890_e_4,
(SPRR1A/1B) M84757(99%)  955_e_11, 692_c_1
Small proline-rich protein 2A/2B 674 bp / 661 bp M20030 (99%) / 874_d_5, 986_e_10, 890_e_4,
(SPRR2A/2B) M21302(99%)  955_e_11, 692_c_1
S100 Calcium-binding protein A7 416 bp M86757 955_e_11, 692_c_1, 100_f_3,
(S100A7) (99%)  950_e_2 (#1, #2, #4–#11)
S100 Calcium-binding protein A6 420 bp M14300 955_e_11, 692_c_1, 100_f_3,
(S100A6) (100%)  950_e_2 (#4, #6, #7, #9, #11)
S100 Calcium-binding protein A4 496 bp M80563 955_e_11, 692_c_1, 100_f_3,
(S100A4) (99%)  950_e_2 (#4, #6, #7, #9, #11)
New-assigned EDC genes
Adenosine deaminase, RNA-specific 2625 bp U18121 950_e_2 (#1–#11),
950_e_2#9 (ADAR1) (99%)  951_f_6, 954_a_11
Cytoskeletal tropomyosin TM30nm 2075 bp X04588 950_e_2 (#2, #4, #7, #9),
(TPM3) (99%) 713_h_12, 951_f_6
26S Proteasome subunit p31 973 bp D38047 950_e_2 (#2–#4, #7–#10),
(PSMD8L) (100%)  951_f_6, 954_a_11
HAX-1, HS1-binding protein 1084 bp U68566 950_e_2 (#2–#4, #7, #9),
(HAX1) (99%) 713_h_12, 951_f_6, 643_h_5
34 / 67 kd Laminin receptor 1025 bp J03799 950_e_2 (#2–#4, #7–#10),
(LAMRL6) (99%)  951_f_6, 643_h_5, 954_a_11
  • All sequenced cDNA inserts originated from the 3′ untranslated region (UTR) of the gene.

This Article

  1. Genome Res. 11: 341-355

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