Northern blot analysis of NICE-1. A Northern blot containing RNA from different cell populations was hybridized with a specific probe for NICE-1. Total RNA (20 μg) in each lane was isolated from the following tissues or cultured cells: (1) human skin; (2) stripped human keratinocytes maintained in medium without calcium (Fischer et al. 1999); (3) stripped human keratinocytes maintained in a proliferative state in medium with 1.8 mM strontium (Praeger et al. 1987); (4) stripped human keratinocytes induced to differentiate in medium with 1.8 mM calcium; (5) HaCaT cells; (6) HeLa cells; (7) SCC4 cells; (8) primary human fibroblasts; (9) primary human melanocytes. The top band is due to cross-reaction with 28S ribosomal RNA.
