Computer-Based Methods for the Mouse Full-Length cDNA Encyclopedia: Real-Time Sequence Clustering for Construction of a Nonredundant cDNA Library

Table 1.

Quality-Check List for 5′ End and 3′ Tag Sequences

N No primer or restriction sites of the linker identified (no insert or inaccuracy sequence)
@ Discrimination of ID-check marker
S Very short sequence (truncated by inaccuracy)
C Contamination (very similar to know E. coli or plasmid sequences)
R Structural RNA contamination
M Mitochondrial genomic sequences
L Long poly A tail
P No or short poly(A) tail
G No or short G tail
  • Used only for 3′ sequences.

  • Used only for 5′ sequences.

This Article

  1. Genome Res. 11: 281-289

Preprint Server