Figure 6.
Detection of chemical synthetic lethality in a blind large-scale test. Clone 12 cells were seeded into 1200 wells in 96-well microplates. Five chemicals were randomly added to three wells each; hygromycin B (H) at 200 μg/mL, MPA (MP) at 25 μg/mL, ribavirin (RI) at 50 μg/mL, mizoribine (MI) at 200 μg/mL, and alanosine at 10 μg/mL. MPA was added in the presence of 200 μM guanine, ribavirin, and mizoribine in 12.5 μM guanine, while alanosine was added together with 60 μM adenine. Plates were read after 28 d, and fluorescent ratios are displayed as in Fig. 5. False-positive wells (F) are also labeled.
