FISH mapping studies in human and apes. Fibroblast cell lines derived from human, pigmy chimp, gorilla, gibbon, and orangutan were used for FISH mapping of the sc11.1 duplication. Two human PAC clones, 641O13 (641) and 743I12 (743), that map to sc11.1b were used to generate fluorescent probes. (A) The 641 probe (red) was used on metaphase chromosome spreads of the human, pigmy chimp, gorilla, and orangutan cell lines. The 743 probe (green) was use on metaphase chromosome spreads of the gibbon cell line. The arrows demarcate chromosome 22 in the human cell line and chromosome 23 in the primate cell lines. (B) The 641 probe (red) was used on metaphase and interphase chromosome spreads. (C) The 743 probe (green) was used on interphase chromosome spreads.
