Expression analysis of cDNA panels. (A) A 172 bp PCR product was synthesized from a panel of human tissue-specific cDNAs (Clontech) using the DGCR6–6F/DGCR6–4R primers. The product was then restriction digested with PvuII, which should cut the DGCR6specific product [(D) digested], 124 bp, but not the DGCR6Lproduct [(U) undigested], 172 bp. The tissue source for the cDNAs is indicated above the lane. The tissues that were analyzed and the number of pooled unrelated individuals used are as follows:Top: brain (3), heart (3), kidney (8), skeletal muscle (9), liver (1), pancreas (21), lung (1), peripheral blood leukocyte (550), ovary (5), thymus (9), colon (20), small intestine (11), spleen (5), prostate (20); Bottom: fetal liver (32), fetal thymus (13), fetal lung (9), fetal skeletal muscle (13), fetal kidney (9), fetal heart (14). (B) Summary table of results determined fromA; most of the tissues contain both digested and undigested products.
