Figure 2.
Bal-31 digestion blot analysis of telomere repeats.Encephalitozoon cuniculi total genomic DNA was digested byBal-31 nuclease for 0, 2.5, 5, 6, 7.5, 8.5 min (lanes1–6, respectively) and subsequently digested withBssHII. This was followed by size-fractionation on agarose gel electrophoresis and Southern blotting onto a nylon membrane. The blot was then hybridized with 32P-labeled telomere repeat oligonucleotides as probes and autoradiographed for 5 h.
