Figure 3.
Assessment of the reverse two-hybrid system as a method to further study Caenorhabditis elegans protein interactions. Reverse two-hybrid system. Each yeast diploid expressing one of the 35 conserved interacting protein pairs from C. elegans was tested to identify those that are sensitive to 5-Fluoroorotic acid (5-FOA). Pairs are arranged in the order described in Table 2. Diploids were replica plated onto both SC-Leu-Trp (A) and SC -Leu, -Trp + 0.2% 5-FOA (B). Twenty-seven of thirty-five pairs (77%) were 5-FOA sensitive.
