Figure 2.
Complementarity of the primers. (A) The internal primer and the poly T primer were analyzed for complementarity to their predicted binding sites on the L1 RNA. The first six nucleotides, numbered from the 3′-hydroxyl, are listed. Nucleotides are highlighted in yellow if they are complementary to the corresponding nucleotide on the L1 RNA. The last row lists the number of complementary nucleotides at each position, out of a possible total of seventeen. (B) The number of matches (r) at each position and the correspondingP-values, representing the likelihood of obtaining r matches or greater by chance alone.
