Organization of the L1 insertion H15/B10-800. The L1 display clone (H15/B10-800) contains the 3′ end of a L1Hs-Ta followed by a 23-bp poly(A) tail and 616-bp of 3′ flanking DNA. GenBank accession no.AC027480 contains a full-length L1Hs-Ta element followed by a 23-bp long poly(A) tail and the proximal 211 bp of 3′-flanking DNA from the H15/B10-800 L1 insertion. More distal 3′ flanking DNA is not homologous to the distal 3′ flanking DNA from H15/B10-800. GenBank accession no.AC026092 is the locus where H15/B10-800 inserted. It contains only the distal 405 bp of the H15/B10-800 3′ flanking DNA. The horizontal arrows indicate the positions of the PCR primers that were used to confirm the structure of the H15/B10-800 integration. The A-rich sequences present at each of the loci between the proximal and distal 3′-flanking DNA are indicated.
