Table 4.
ORFs of Synechocystis sp. Strain PCC 6803 Inactivated in This Work
| ORF | ORF coordinates | Oligonucleotides coordinates | Inserted cassette and insertion site |
| slr1145 | 809450–810658 | 809342–809362, 810371–810351 | C.K3 or C.C1, NcoI (Klenow-filled) |
| sll1270 | 1116021–1114429 | 1116052–1116033, 1115052–1115072 | C.C1,KpnI |
| slr1735 | 1317712–1318470 | 1317628–1317647, 1318781–1318761 | C.K3 or C.C1,SmaI |
| slr0949 | 2017104–2017964 | 2017139–2017158, 2018372–2018353 | C.K3,HpaI |
| slr1881 | 1239490–1240212 | 1239564–1239583, 1240494–1240475 | C.K3,StuI |
| sll0146 | 2192051–2190933 | 2191877–2191858, 2190922–2190941 | C.K3, SmaI |
| sll1102, sll1103 | 1914652–1914113, 1914113–1912776 | 1914799–1914779, 1913403–1913423 | C.K3, 2 BamHI sites |
| sll1104 | 1912786–1911896 | 1912674–1912654, 1911573–1911593 | C.K3, XbaI |
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↵The names and chromosome coordinates (Kaneko et al. 1996) of the inactivated ORFs are indicated in the first and second columns, respectively. The coordinates of the oligonucleotides used for PCR amplification of those ORFs are indicated in the third column. The inserted antibiotic resitance-encoding gene cassette and the restriction endonuclease insertion site(s) are indicated in the last column.
