Identification of Genes Encoding Amino Acid Permeases by Inactivation of Selected ORFs from the Synechocystis Genomic Sequence

Table 1.

Amino Acid Transport Activities of Synechocystis sp. Strain PCC 6803 and Some Mutants

Substrate amino acid Amino acid transport in strain PCC 6803 (nmol · min−1 · mg Chl−1) Amino acid transport (% of the wild-type activity)
CSMJ36 natD (slr0949) CSMJ37 natE (slr1881) CSMJ41 natC (sll0146) CSMJ7 bgtB (sll1270) CSMJ8 bgtA (slr1735) CSMJ11 bgtB bgtA (sll1270 slr1735) CSMJ24 natB bgtB (slr0559 sll1270) CSMJ26 natB bgtA (slr0559 slr1735) CSMJ30 natA bgtB (slr0467 sll1270) CSMJ32 natA bgtA (slr0467 slr1735)
Basic
 Arg 426.7 90 92 86 3 3 3 <1 3 1 1
 His 6.2 n.d. n.d. n.d. 9 12 10 2 2 5 2
 Lys 52.8 95 88 87 <1 <1 <1 <1 <1 <1 <1
Neutral
 Ala 10.2 1 1 1 80 91 97 <1 <1 1 <1
 Gln 5.9 98 86 89 19 21 22 2 2 3 3
 Gly 9.4 1 1 <1 80 93 89 1 1 2 1
 Leu 7.7 2 1 1 n.d. n.d. n.d. n.d. n.d. n.d. n.d.
 Pro 6.1 1 1 1 79 99 85 <1 <1 <1 1
 Ser 7.0 2 2 2 78 80 96 2 3 2 1
Acidic
 Glu 60.9 87 98 71 99 74 97 89 95 85 84
  • Data are the mean of 6–12 determinations with similar results (within 20% of the mean).

  • Two to four determinations were performed for each mutant, with similar results. For simplicity, the data are presented as a percent of the wild-type activity.

  • n.d., not determined.

This Article

  1. Genome Res. 11: 2034-2040

Preprint Server