TIGR Mouse BAC End Sequencing Efforts (June 27, 2001)
| Library | RPCI-23 | RPCI-24 | Total |
| Sequencing success rate (%) | 83 | 79 | 81 |
| Mouse BAC end sequences (mBESs) | 274277 | 174957 | 449234 |
| Clones with ≥1 end and coverage | 154795, 10X | 102524, 5.3X | 257318, 15.3X |
| Paired-end clones and coverage | 119483, 77%, 7.9X | 72433, 71%, 3.7X | 191916, 75%, 11.6X |
| Edited read length, avg. ± S.D. bp | 466 ± 162 | 515 ± 188 | 485 ± 174 |
| Total bases, Mb | 128 | 90 | 218 |
| Avg. Q20 bases after trimming, bp | 387 ± 154 | 438 ± 179 | 406 ± 166 |
| Repeat contents, %mBES and %bases | 63 & 36.5 | 66 & 37.5 | 64 & 37 |
| Clones with ≥100 unique Q20 bases | 65% | 66% | 66% |
| %mBESs matching mouse ESTs | 2.2 | 4.5 | 3 |
| Clone tracking accuracy | >95% | >95% | >95% |
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↵Percent of sequencing attempts that yielded reads with an overall error rate <2.5%, an edited read length >100 bp, and free of E. coli and vector sequences.
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↵Clones with at least one end sequence. Clone coverage was calculated assuming an average insert size of 197 kb for PRCI-23 library and 155kb for PCRI-24 library, and a genome size of 3 Gb.
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↵Clones with both T7 and Sp6 end sequences (pairs). Clone coverage by pairs was calculated as described above. Total number of pairs, % of pairs in the database, and their coverage are shown.
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↵Read length after sequences were trimmed. The average and standard deviation (S.D.) are shown.
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↵Average number of bases with phred QV ≥ 20 per sequence after sequences were trimmed. The average and S.D. are shown.
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↵Percent of mBESs that contained repeats and percent of bases that were repeats, analyzed by RepeatMasker.
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↵Percent of paired-end clones that had at least one end with ≥100 bp contiguous unique Q20 bases.
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↵Percent of mBESs that were associated with the correct clone identifiers.
