A Human cDNA Expression Library in Yeast Enriched for Open Reading Frames

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Figure 5.
Figure 5.

Purification and cleavage of the GAPDH-HIS3 fusion protein. The fusion protein was expressed in S. cerevisiae GRF18 and purified using Ni-NTA agarose. Proteins were visualized by Coomassie staining. (Lane 1) Prestained Bio-Rad broad range marker; (lane2) cell lysate; (lane 3) flow-through; (lanes4–6: wash1-3; (lanes 7,8) eluate 1 and 2; (lane 9) cleavage of the C-terminally fused HIS3 protein by PreScission Protease.

This Article

  1. Published in Advance September 20, 2001, doi: 10.1101/gr.181501 Genome Res. 11: 1730-1735

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