Figure 4.
Expression of different inserts from HFBr cDNA clones in the ORF-selecting vector pYEXTSH3. Proteins separated by SDS-PAGE (100 μg each lane) were detected from crude cell extracts by immunoblotting with an anti-RGS-His6 antibody. (Lanes1–5) HIS3 fusion proteins from selected cDNA expression clones 001-B2, 001-H1, 001-F1, 001-F2, and 001-E3; (lanes6–9) HIS3 from pYEXT2His3, pYEXTSH3 without insert (negative control), pYEXTGnH3 (GAPDH not in-frame), and pYEXTGH3 (GAPDH in-frame, positive control). The positions of the prestained Bio-Rad broad range marker are indicated on the left.
