A Comparative Molecular Analysis of Developing Mouse Forelimbs and Hindlimbs Using Serial Analysis of Gene Expression (SAGE)

Table 4.

A List of Genes Known to be Differentially Expressed between Forelimbs and Hindlimbs

Gene SAGE tag Tag count
forelimb hindlimb
Pitx1 TACGTCTATT 0 26
Pitx1(IP) TCGCCGGGCG 0 14
Tbx4 GACATTTTGT 0 9
Tbx5 TTCCCCGATT 3 0
Hoxc9 TACGGCTCGC 0 2
Hoxc10 TAGCTTCCTT 0 4
CAAAGTTGAG 0 5
Hoxc11 TGCGTGAGTG 0 1
Hoxd9 GGTTGGAAAA 0 0
  • This SAGE tag appears to represent a cDNA generated from an internally primed poly(A) rich region of Pitx1.Further analysis showed that this SAGE tag also matched at the 11th base-pair relative to the Pitx1 sequence data (GI:1616804,CATG at 1256 bp).

  • This SAGE tags were identified by cloning and sequencing the 3′ ends of these genes (see Methods for details).

  • Obtained from an analysis of GenBank sequence data (GI:51413). There are two different poly(A) signals that would generate the two different SAGE Tags listed here, the second of which was generated from an ATTAAA poly(A) signal (Peterson et al. 1992).

  • Obtained from the sequence presented in Figure 1 of Hostikka and Capecchi (1998).

  • A tag with a 1 bp mismatch was observed 1 and 0 (GGTTGGAAGA) and may represent Hoxd9.

This Article

  1. Genome Res. 11: 1686-1698

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