A Comparative Molecular Analysis of Developing Mouse Forelimbs and Hindlimbs Using Serial Analysis of Gene Expression (SAGE)

Table 3.

Hox Genes Identified in the Forelimb and Hindlimb SAGE Libraries

Gene SAGE tag Tag count
forelimb hindlimb
Hoxa1 TCTGTAATAA 0 1
Hoxa7 AAGTGGAAGA 1 1
Hoxa9 AAACTGCTCT 5 2
Hoxa10 CATAAAAGGG 13 10
Hoxa11 TGAAATAATA 0 1
Hoxa11,antisense1 CAATTGAGGC 9 7
Hoxa11,antisense1 CATCAGGGTA 0 4
Hoxa13 GTGGATTAAC 2 4
Hoxb8 CGCGCTGTGA 0 1
Hoxc9 TACGGCTCGC 0 2
Hoxc10 TAGCTTCCTT 0 4
CAAAGTTGAG 0 5
Hoxc11 TGCGTGAGTG 0 1
Hoxd10 TTTCTGAAAA 1 0
Hoxd11 AGTCACTGTC 17 15
Hoxd13 GGCCTCTCAG 6 3
  • Unless otherwise noted, all SAGE tags in this table were initially identified by the ehmtag-mapping method and subsequently verified manually.1There are numerous polyadenylated antisense transcripts produced from this locus (Hsieh-Li et al. 1995). Both of these SAGE tags are valid matches to different antisense transcripts.

  • The SAGE tags for these genes were identified by cloning and sequencing the 3′ ends of the genes.

  • Obtained from an analysis of GenBank sequence data (GI:51413). There are two different poly(A) signals that would generate the two different SAGE tags listed here, the second of which was generated from an ATTAA poly(A) signal and poly(A) rich region 1 kb upstream (at 5752 bp) of the reported putative poly(A) signal (Peterson et al. 1992).

  • Obtained from the sequence presented in Figure 1 of Hostikka and Capecchi (1998).

  • This SAGE tag was identified from a genomic clone (see Methods for details).

This Article

  1. Genome Res. 11: 1686-1698

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