A Comparative Molecular Analysis of Developing Mouse Forelimbs and Hindlimbs Using Serial Analysis of Gene Expression (SAGE)

Table 2.

Distribution of SAGE Tags Matching Genes or ESTs, Sorted by Abundance Class

Copies per cell Percent abundance Range of tag frequency Matching gene EST No match Total
>100 >0.27    >382 18 1 2 21
50–100 0.132–0.27 183–363 51 13 7 71
5–50 0.0135–0.132 19–180 440 199 162 801
1–5 0.0025–0.0135 4–18 1,280 2,077 1,570 4,927
0.3–1 0.0015–0.0022 2–3 776 2,195 3,463 6,434
<0.3 <0.00075 1   775 3,563 19,708 24,046
All transcripts 3,711 8,296 24,293 36,300
(10.2%) (22.9%) (66.9%)
  • This table represents information calculated from the sum of both SAGE libraries (136,856 total tags representing 36,300 unique transcripts.)

  • Unless otherwise noted, ranges are given as > the first value and ≤ the second value.

  • Copies per cell was calculated assuming, or average, 500,000 transcripts in a cell (Hastie and Bishop 1976).

  • If a tag matches a gene and an EST, it was counted only as a gene. See Methods for tag-to-UniGene cluster matching algorithm.

This Article

  1. Genome Res. 11: 1686-1698

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