Hybridization of genomic DNA to gridded small-insert library. (A) Phylogenetic tree demonstrating relationship of species for which genomic DNA was used as probes for the small insert library. The tree is based on data from Hilton and Gaut (1998). The branch lengths indicate time since divergence with time estimates denoted above. (B) Examples of hybridization patterns for 108 clones spotted in a nonregular duplicate arrangement. The center spot of each 5×5 grid contains λ DNA, which was not included in the probe mixture. (C) Pairwise comparisons of hybridization data to the cne1g library for the Zea probes: Z. luxurians,Z. diploperennis, Z. mays (B73), and Z. perennis. Each clone is plotted according to hybridization intensity in two species. Clones containing knob repeats are indicated by open triangles. The correlation coefficient R is shown either for all clones or for those containing knob repeats only.
