Whole-mount expression of atpα1A1, atpα1B1 andatpα2 during zebrafish development. Parallel expression analysis of zebrafish sodium pump catalytic subunits at the 17 somite, 24 hpf, 48 hpf, and 96 hpf stages. At the 17 somite stage,atpα1A1 is expressed in the intermediate mesoderm and at lower levels in the otic placode (A,D);atpα1B1 is expressed in the otic placode, optic cup and throughout the brain (B,E); atpα2 is expressed in the somites (C,F). At 24 hpf,atpα1A1 expression is seen in the nephric duct (G) and lower levels in the otic vesicle; atpα1B1 is expressed in the brain and nephric duct (H), whereas atpα2expression remains exclusive to the muscle lineage (I). At 48 hpf, atpα1A1 expression is detected in the otic vesicle and nephric duct (J); atpα1B1 expression remains in the brain, but is now also seen in the fin buds (K) and the heart (asterisk in L); atpα2 is also detected in the fin buds (M). (N,O) Expression ofatpα1A1 and atpα1B1 at 96 hpf, note the absence of atpα1A1 expression in the liver and the strong expression of atpα1B1 in the brain. (im) Intermediate mesoderm; (op) otic placode; (oc) optic cup; (nd) nephric duct; (ov) otic vesicle; (fb) fin bud; (pt) pronephric tubule; (g) gut; (lv) liver.
