Table 1.
Amplimers Developed from Direct Sequencing of BAC, PAC, and YAC Ends
| Sequence | GenBank acc. no. | F (5′-3′) | R (5′-3′) |
| RG1-F | AJ286911 | AGTTTCCTCACAGTGCATGC | CAGGCTCAGATGCAGGTAC |
| RG1-R | AJ286912 | TTCACTTTTCCTGGAGCTGC | AGACCTAGGACACAAGGATG |
| 10d9-R | AJ277870 | CTGAAAGATATCAGCACCTTG | TGGTCATAGCCATCCATGTAG |
| 16o12-T7 | AJ400818 | GAGAGATATTGGTCAAAGGG | CCTCTCAGCAAATTTAAGCATC |
| 30c6-F | AJ286924 | CTTATGTAACACAGTATCTGTGG | GGTCTTATAGTCTTCAGTGG |
| 30c6-R | AJ286925 | TCAGAGTCACCCATCTAAGG | GGGAGCCACTTTAAGCTCC |
| 104F6-F | AJ400820 | GGGCAGTAACAGGAACAGG | TTTATCTGCGTGGCCTTTGC |
| 104F6-R | AJ400819 | TTACTCTGAATTCTAAATTTCC | TTGCCCAGCTGGGTTCTGG |
| 167c4-R | AJ286928 | CATCTCCAGCTCAACCTCATAGG | ATGGGAGACAGTGCCAGG |
| 309j8-T7 | AJ400817 | TAGCAGGTGACAGCTCCATG | GTTAAAAACTAAGACGCAAATGC |
| 330n12-T7 | AJ276449 | CGAAATGAGACTGGAAGTTGGC | CTTGTTCAGGAACAAACCAGCC |
| 330n12-SP6 | AJ276448 | TCAGAACTGAAGTGTATGGATC | AGTTATGTTCCTCACTTGATGC |
| 463e12-F | AJ286921 | TCACTGTGACTTCTCACTGC | GGAATAGGAAGTCATATCAGG |
| 463e12-R | AJ286922 | GCCCTGCTCCATGACCTG | CTCTGGCATTCTCCTCAAGC |
| REP471 | AJ286913 | GCACTTAAAGCTCTTTACTGG | TGGTTTTCAATGATTGCAAGTC |
| 567n19-F | AJ286926 | CAGGACTGCAATTCCTCCAG | ATACTGGAATTGCTCAGAGG |
| 576n19-R | AJ286927 | GACTTCTGGATGGGGTATGG | GAGACAGATCTACTAGGTCC |
| 10k5-T7 | AJ286894 | GCACACTGCAGCAGAGGT | GTGTCCTGGGACTGCCAAG |
| 10k5-SP6 | AJ286895 | CTTCTCAGGCAACCCTACC | CTCATCTCCTGGCTCACTG |
| 68e5-T7 | AJ286896 | TTAGGACTAGGTTGTTCTCAC | TTGGGTACCTGTCACTCTG |
| 68e5-SP6 | AJ286897 | CATTGAACAACATGTACAAGG | GCCCTTGGTAAAAGAGTGAG |
| 76a14-T7 | AJ286899 | ACAATTCTGATGCCAGTGTTC | GAGGAGTCATAGCAGCCAC |
| 76a14-SP6 | AJ286898 | AGTTCTACAAGAGCAGGGAC | AATCTTCTCTAGCTACTCAG |
| 80n21-T7 | AJ286907 | ATAGAAACTTCAGGTCCTTGC | TTGCCTCCATCCTTAGAAGC |
| 80n21-SP6 | AJ286906 | CTTACAGTAGTCTTCTGGATC | CCAAGTTCAGCTGAGGCTG |
| 103k15-T7 | AJ286903 | TACTGAGAGGTGACATGCC | CTGAAGGCACCAACAGATGC |
| 103k15-SP6 | AJ286902 | TCGCCCAGTCTCCTCATG | TCCCCTGAACCTATAAGC |
| 126h9-T7 | AJ277872 | GAAGCTCTCCTGAACCCTC | GTTCATTTAATCCTCACCATCAC |
| 142g11-T7 | Designed from pDJ443n8 (AC004587) | GTTCCACTTCAAACTTCATGG | AGACAGGGTTAACAGCTACG |
| 142g11-SP6 | AJ286900 | TTACTGAAATCAATTACTCAC | CTACTTTGGGGACACTATCC |
| 216i14-T7 | AJ286905 | ATTCTCATCCAAATCCACCTG | GTGTTGTGAGAGACCACGTG |
| 216i14-SP6 | AJ286904 | GTATCCCAAATCCCGTCTTG | GGAAAATCTTTCTTGGAGATC |
| 223e10-SP6 | AJ286916 | AATGGAATTAGAAATTGCATAGAG | GTAAGTTGTAAACTTAGGTTGATG |
| 252a23-T7 | AJ286909 | ACCAAAGGGGCAAAGAGCAG | CGATTTTCTTAGGGTGTGTATC |
| 252a23-SP6 | AJ286910 | ACCTTGGAAGCCCTTAATCC | TCCTTATATGAGGCTGGTCC |
| 802_b_4-Left | AJ286893 | GTCTTGCTAGAGCTGAGG | CATGTTGACCACGACCAG |
| 802_b_4-Right | AJ286892 | GAATTCTCTGGCCCAGCTGAC | CCTTCACTTAAAAACAGCC |
